Materials and Methods:
Blood samples were sent to our laboratory and extracted to yield RNA (Qiagen, Qiasymphony Dsp Virus/Patogen Midi Kit,Germany) and had the RNA quantitation with Rotor-Gene RG-3000 (Corbett Research, Qiagen). Genotyping of samples was carried out with Real Time PCR Reagents Kit (Genome Diagnostics, India).
Results:
In our multivariable analysis, while evaluating the association of the different genotypes with demographic data, we find a notable difference in genotype distribution among genders and it was obviously seen that genotype 1 was common among older patients, while genotype 3 was common among younger subjects.
Conclusion:
This study demonstrated the existence of genotype 1 patients highly prevalant (%60) in our study. Although there was no statistically significant relationship between genotype distrubition and HCV RNA load (p>0.005) and AST levels, ALT levels in group of genotype 3 were significantly higher (p=0.0001).Genotype 1 patients were 60% of all and the results of prevalence rates are consistent with other literature in Turkey.
Keywords: HCV, genotype, real time PCR